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Image Search Results
Journal: Frontiers in Plant Science
Article Title: Kinetic analysis of the interactions between plant thioredoxin and target proteins
doi: 10.3389/fpls.2013.00508
Figure Lengend Snippet: Interaction between the oxidized form of the MDH and Trx h 1 CS proteins. (A) To investigate the association and dissociation phases, the indicated concentrations of MDH were injected for 600 s, followed by injection by buffer for additional 600s (left panel) and the SPR signals were recorded. Fitted curves are indicated by the black lines on the SPR sensorgram. The association and dissociation phases are indicated by bars on the sensorgram. The arrow indicates the time when 5 mM DTT was injected (right panel). (B) Mixed disulfide intermediate complexes formed by the Trx h 1 CS and oxidized MDH were separated by gel filtration chromatography (TSK-G2000SW XL ) (black trace). The intermediate complexes (orange colored portion) were fractionated and then applied to the same column chromatography after incubation for the indicated period in the presence (blue trace) or the absence (red trace) of 10 mM NEM.
Article Snippet:
Techniques: Injection, Filtration, Chromatography, Column Chromatography, Incubation